CASK antibody - 150 103

CASK is a MAGUK family protein of the pre-synaptic active zone

Rabbit polyclonal purified antibody

Cat. No.: 150 103

Amount: 50 µg

Price: $455.00

Cat. No. 150 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 318 to 415 from rat CASK (UniProt Id: Q62915)
Reactivity	Reacts with: rat (Q62915), mouse (O70589). Other species not tested yet.
Data sheet	150_103.pdf

Cat. No.: 150 103

Amount: 50 µg

Price: $455.00

Background

CASK, like PSD 95, belongs to the family of membrane associated guanylate kinase homologues (MAGUKs) but contains an additional N-terminal CaM kinase-like domain.
It has been shown to interact with Mint and Veli via its N-terminal domains. PDZ domains are present in all three proteins of the complex. They are free to recruit other proteins like neurexins and syndecans. The complex of CASK, Velis and Mint is conserved across kingdoms and has also been observed in C. elegans.

1 ) Tabuchi K et al., J. Neurosci. (2002)
2 ) Sanford JL et al., Muscle Nerve (2004)
3 ) Leonoudakis D et al., J. Biol. Chem. (2004)
4 ) Zhang Y et al., FEBS Lett. (2001)
5 ) Butz S et al., Cell (1998)
6 ) Missler M et al., J. Neurochem. (1998)

Protocols

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