CASK antibody - 150 002

CASK is a MAGUK family protein of the pre-synaptic active zone

Rabbit polyclonal antiserum

Cat. No.: 150 002

Amount: 200 µl

Price: $355.00

Cat. No. 150 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 337 from rat CASK (UniProt Id: Q62915)
Reactivity	Reacts with: rat (Q62915), mouse (O70589), hamster. Other species not tested yet.
Data sheet	150_002.pdf

References for CASK - 150 002

The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels.
Morciano M, Beckhaus T, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2009) 1083: 662-75. 150 002 WB

Cat. No.: 150 002

Amount: 200 µl

Price: $355.00

Background

CASK, like PSD 95, belongs to the family of membrane associated guanylate kinase homologues (MAGUKs) but contains an additional N-terminal CaM kinase-like domain.
It has been shown to interact with Mint and Veli via its N-terminal domains. PDZ domains are present in all three proteins of the complex. They are free to recruit other proteins like neurexins and syndecans. The complex of CASK, Velis and Mint is conserved across kingdoms and has also been observed in C. elegans.

1 ) Tabuchi K et al., J. Neurosci. (2002)
2 ) Sanford JL et al., Muscle Nerve (2004)
3 ) Leonoudakis D et al., J. Biol. Chem. (2004)
4 ) Zhang Y et al., FEBS Lett. (2001)
5 ) Butz S et al., Cell (1998)
6 ) Missler M et al., J. Neurochem. (1998)

Protocols

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