Synaptic Systems

Cat. No.: 262 003

Amount: 50 µg

Price: $375.00

Cat. No. 262 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 gallery IP: yes ICC: not tested yet IHC: yes IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 18 to 107 from mouse CAPS1 (UniProt Id: Q80TJ1)
Reactivity	Reacts with: rat (Q62717), mouse (Q80TJ1). Other species not tested yet.
Specificity	Specific for CAPS 1, no cross-reactvity to CAPS 2 K.O. validated
Data sheet	262_003.pdf

References for CAPS1 - 262 003

RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission.
Shumate KM, Tas ST, Kavalali ET, Emeson RB
Journal of neurochemistry (2021) 1582: 182-196. 262 003 WB; tested species: mouse

Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells.
Vogl C, Cooper BH, Neef J, Wojcik SM, Reim K, Reisinger E, Brose N, Rhee JS, Moser T, Wichmann C
Journal of cell science (2015) 1284: 638-44. 262 003 IHC

Cat. No.: 262 003

Amount: 50 µg

Price: $375.00

Background

The Ca²⁺-dependent activator protein for secretion (CAPS) regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites.
Two differen isoforms CAPS 1/CADPS 1 and CAPS 2/CADPS 2 that are mainly expressed in brain have been identified in mammals. Both have been shown to be essential components of the synaptic vesicle priming machinery.

1 ) Sadakata T et al., J. Biol. Chem. (2010)
2 ) Speidel D et al., Cell Metab. (2008)
3 ) Sadakata T et al., J. Histochem. Cytochem. (2007)
4 ) Jockusch WJ et al., Cell (2007)
5 ) Sadakata T et al., J. Comp. Neurol. (2006)
6 ) Speidel D et al., Neuron (2005)

Protocols

Standard protocol

IHC

Other products for this target

CAPS1 antibody - 262 003 K.O.

References for CAPS1 - 262 003