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Ca2+ channel R-type alpha-1E antibody - 152 404

Guinea pig polyclonal antiserum
Cat. No.: 152 404
Amount: 100 µl
Price: $365.00
Cat. No. 152 404 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type α-1E (Cav2.3) (UniProt Id: Q07652)
Reactivity Reacts with: rat (Q07652), mouse (Q61290).
Other species not tested yet.
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel R-type alpha-1E aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended:

Data sheet 152_404.pdf
Cat. No.: 152 404
Amount: 100 µl
Price: $365.00
Background
Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1E isoform occurs in VGCCs of the R-type which belongs to the high voltage activated group (hva).