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Ca2+ channel N-type alpha-1B antibody - 152 305

Guinea pig polyclonal purified antibody
Cat. No.: 152 305
Amount: 50 µg
Price: $460.00
Cat. No. 152 305 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294)
Reactivity Reacts with: rat (Q02294), mouse (O55017).
Other species not tested yet.
Matching control protein/peptide 152-3P
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel N-type alpha-1B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires mild fixation.

Data sheet 152_305.pdf

References for Ca2+ channel N-type alpha-1B - 152 305

Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration (2022) 111: 31. 152 305 ICC; tested species: mouse
BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).
Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S
Frontiers in molecular neuroscience (2017) 10: 346. 152 305 ICC; tested species: mouse
Cat. No.: 152 305
Amount: 50 µg
Price: $460.00
Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration (2022) 111: 31. 152 305 ICC; tested species: mouse
BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).
Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S
Frontiers in molecular neuroscience (2017) 10: 346. 152 305 ICC; tested species: mouse
Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).