Cat. No. 152 305 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: yes gallery |
Immunogen | Recombinant protein corresponding to AA 2056 to 2336 from rat Ca2+ channel N-type α-1B (Cav2.2) (UniProt Id: Q02294) |
Reactivity |
Reacts with: rat (Q02294), mouse (O55017). Other species not tested yet. |
Matching control protein/peptide | 152-3P |
Remarks |
WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel N-type alpha-1B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
Data sheet | 152_305.pdf |
Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).