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Home > Products > 334 003

Ca2+ channel L-type alpha-1C antibody - 334 003

Rabbit polyclonal purified antibody
Cat. No.: 334 003
Amount: 50 µg
Price: $455.00
Cat. No. 334 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1901 to 2169 from rat Ca2+ channel L-type α-1C (Cav1.2) (UniProt Id: P22002)
Reactivity Reacts with: rat (P22002), mouse (Q01815).
Other species not tested yet.
Remarks

WB: Due to its large size, Ca-channels require special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with NuPage TRIS-acetate gels from Invitrogen.
Non-boiled samples are recommended.
Data sheet 334_003.pdf

References for Ca2+ channel L-type alpha-1C - 334 003

Adaptive Mechanisms of Somatostatin-Positive Interneurons after Traumatic Brain Injury through a Switch of α Subunits in L-Type Voltage-Gated Calcium Channels.
Ihbe N, Le Prieult F, Wang Q, Distler U, Sielaff M, Tenzer S, Thal SC, Mittmann T
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 334 003 WB, IHC; tested species: mouse
Adaptive Mechanisms of Somatostatin-Positive Interneurons after Traumatic Brain Injury through a Switch of α Subunits in L-Type Voltage-Gated Calcium Channels.
Ihbe N, Le Prieult F, Wang Q, Distler U, Sielaff M, Tenzer S, Thal SC, Mittmann T
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 334 003 WB, IHC; tested species: mouse
Cat. No.: 334 003
Amount: 50 µg
Price: $455.00
Adaptive Mechanisms of Somatostatin-Positive Interneurons after Traumatic Brain Injury through a Switch of α Subunits in L-Type Voltage-Gated Calcium Channels.
Ihbe N, Le Prieult F, Wang Q, Distler U, Sielaff M, Tenzer S, Thal SC, Mittmann T
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 334 003 WB, IHC; tested species: mouse
Adaptive Mechanisms of Somatostatin-Positive Interneurons after Traumatic Brain Injury through a Switch of α Subunits in L-Type Voltage-Gated Calcium Channels.
Ihbe N, Le Prieult F, Wang Q, Distler U, Sielaff M, Tenzer S, Thal SC, Mittmann T
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 334 003 WB, IHC; tested species: mouse
WB
IHC
Background
Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1C subunit occurs in VGCCs of the L-type which belongs to the high voltage activated group (hva).
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