BoNT antibody - 158 002

BoNT is a neurotoxin inhibiting neurotransmitter release

Rabbit polyclonal antiserum

Cat. No.: 158 002

Amount: 200 µl

Price: $355.00

Cat. No. 158 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 422 from Clostridium botulinum BoNT (UniProt Id: Q00496)
Specificity	Specific for BoNT E.
Data sheet	158_002.pdf

Cat. No.: 158 002

Amount: 200 µl

Price: $355.00

Background

The neurotoxins of Clostridium botulinum BoNTs belong to the most potent protein toxins. These Zinc proteases elicit paralysis by cleaving SNARE proteins like SNAP 25, Syntaxin 1 and Synaptobrevin which mediate the fusion of synaptic vesicles with the presynaptic membrane.
There are seven known serotypes of BoNTs (A to G) with different cleavage properties. BoNTs are translated as ~150 kDa single-chain, non-toxic precursor proteins which are cleaved into a catalytic light chain (LC) and a heavy translocation chain (HCT).

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