Synaptic Systems

Cat. No.: 256 003

Amount: 50 µg

Price: $375.00

Cat. No. 256 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 610 to 863 from mouse BAIAP3 (UniProt Id: Q80TT2)
Reactivity	Reacts with: rat, mouse (Q80TT2). Other species not tested yet.
Specificity	Specific for BAIAP 3. K.O. validated
Data sheet	256_003.pdf

References for BAIAP3 - 256 003

Dense cholinergic projections to auditory and multisensory nuclei of the intercollicular midbrain.
Noftz WA, Beebe NL, Mellott JG, Schofield BR
Hearing research (2021) 411: 108352. 256 003 WB, ICC; KD verified; tested species: mouse

BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.
Zhang X, Jiang S, Mitok KA, Li L, Attie AD, Martin TFJ
The Journal of cell biology (2017) 2167: 2151-2166. 256 003 WB, ICC; KD verified; tested species: bon cells

Cat. No.: 256 003

Amount: 50 µg

Price: $375.00

Background

BAIAP 3 (BAI 1-associated protein 3), also known as BAP 3, is a transmembrane protein that contains two C2 domains, one MHD1 domain and one MHD2 domain. It has the same domain structure as Munc 13 proteins and is predominantly expressed in brain. BAIAP 3 is also a known interaction partner of BAI 1, a member of the secretin-receptor family.
Recently BAIAP 3 has been discussed as a marker protein for prognostic evaluation of ependymomas, that are primary tumors of the central nervous system.

1 ) Lauridsen JB et al., Neurosci. Res. (2011)
2 ) Palm T et al., Cancer (2009)
3 ) Palmer RE et al., Cancer Cell (2002)
4 ) Koch H et al., Biochem. J. (2000)

Protocols

BAIAP3 antibody - 256 003 K.O.

References for BAIAP3 - 256 003