beta-Actin antibody - 251 005

beta-Actin is a major constituent of the cytoskeleton

Guinea pig polyclonal purified antibody

Cat. No.: 251 005

Amount: 50 µg

Price: $460.00

Cat. No. 251 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 gallery IP: yes ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity	Reacts with: rat (P60711), mouse (P60710). Other species not tested yet.
Specificity	May cross-react to α- and γ-actin due to sequence homology.
Remarks	ICC: Methanol fixation is recommended.
Data sheet	251_005.pdf

Cat. No.: 251 005

Amount: 50 µg

Price: $460.00

ICC

Background

The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.

1 ) Bassell GJ et al., J. Neurosci. (1998)
2 ) Ng SY et al., Nucleic Acids Res. (1989)
3 ) Nudel U et al., Nucleic Acids Res. (1983)
4 ) Czosnek H et al., EMBO J. (1983)

Protocols

Standard protocol

ICC

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