beta-Actin antibody - 251 005

beta-Actin is a major constituent of the cytoskeleton

Guinea pig polyclonal purified antibody

Cat. No.: 251 005

Amount: 50 µg

Price: $460.00

Cat. No. 251 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 gallery IP: yes ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity	Reacts with: rat (P60711), mouse (P60710). Other species not tested yet.
Specificity	May cross-react to α- and γ-actin due to sequence homology.
Remarks	ICC: Methanol fixation is recommended.
Data sheet	251_005.pdf

Cat. No.: 251 005

Amount: 50 µg

Price: $460.00

ICC

Background

The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.

1 ) Bassell GJ, The Journal of neuroscience : the official journal of the Society for Neuroscience (1998) 181: 251-65.
2 ) Ng SY, Nucleic acids research (1989) 172: 601-15.
3 ) Nudel U, Nucleic acids research (1983) 116: 1759-71.
4 ) Czosnek H, The EMBO journal (1983) 211: 1977-9.

Protocols

Standard protocol

ICC

Other products for this target