Synaptic Systems - beta-Actin

Cat. No.: 251 003

Amount: 100 µg

Price: $375.00

Cat. No. 251 003	100 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 gallery IP: yes ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Reactivity	Reacts with: rat (P60711), mouse (P60710), chicken, zebrafish. Other species not tested yet.
Specificity	May cross-react to α- and γ-actin due to sequence homology.
Remarks	ICC: Methanol or mild PFA fixation (2%).
Data sheet	251_003.pdf
Documents	Science Exchange validation report

References for beta-Actin - 251 003

Membrane mechanics dictate axonal pearls-on-a-string morphology and function.
Griswold JM, Bonilla-Quintana M, Pepper R, Lee CT, Raychaudhuri S, Ma S, Gan Q, Syed S, Zhu C, Bell M, Suga M, et al.
Nature neuroscience (2024) : . 251 003 WB; tested species: mouse

S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 251 003 WB; tested species: rat

Deletion of hippocampal Glucocorticoid receptors unveils sex-biased microRNA expression and neuronal morphology alterations in mice.
Tejos-Bravo M, Oakley RH, Whirledge SD, Corrales WA, Silva JP, García-Rojo G, Toledo J, Sanchez W, Román-Albasini L, Aliaga E, Aguayo F, et al.
Neurobiology of stress (2021) 14: 100306. 251 003 WB; tested species: mouse

The Synaptic Vesicle Priming Protein CAPS-1 Shapes the Adaptation of Sensory Evoked Responses in Mouse Visual Cortex.
Nestvogel DB, Merino RM, Leon-Pinzon C, Schottdorf M, Lee C, Imig C, Brose N, Rhee JS
Cell reports (2020) 3010: 3261-3269.e4. 251 003 WB; tested species: mouse

A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, et al.
Nature communications (2020) 111: 2729. 251 003 WB; tested species: rat

Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 251 003 WB; tested species: frog

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 251 003 WB; tested species: human

Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 251 003 WB; tested species: mouse

The α-subunit of the trimeric GTPase Go2 regulates axonal growth.
Baron J, Blex C, Rohrbeck A, Rachakonda SK, Birnbaumer L, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2013) 1246: 782-94. 251 003 WB

Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.
Whitemarsh RC, Strathman MJ, Chase LG, Stankewicz C, Tepp WH, Johnson EA, Pellett S
Toxicological sciences : an official journal of the Society of Toxicology (2012) 1262: 426-35. 251 003 WB

The receptor for advanced glycation end products and its ligands' expression in OVE26 diabetic sciatic nerve during the development of length-dependent neuropathy.
Zglejc-Waszak K, Schmidt AM, Juranek JK
Neuropathology : official journal of the Japanese Society of Neuropathology (2022) : . 251 003 IHC; tested species: mouse

Cat. No.: 251 003

Amount: 100 µg

Price: $375.00

ICC

Background

The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.

1 ) Bassell GJ et al., J. Neurosci. (1998)
2 ) Ng SY et al., Nucleic Acids Res. (1989)
3 ) Nudel U et al., Nucleic Acids Res. (1983)
4 ) Czosnek H et al., EMBO J. (1983)

Protocols

Standard protocol

ICC

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beta-Actin antibody - 251 003

References for beta-Actin - 251 003