ASPEP antibody - 296 003

ASPEP is an aminopeptidase with specificity towards an acidic amino acid at the N-terminus

Rabbit polyclonal purified antibody

Cat. No.: 296 003

Amount: 50 µg

Price: $375.00

Cat. No. 296 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 195 to 213 from mouse AS (UniProt Id: Q9Z2W0)
Reactivity	Reacts with: rat (Q4V8H5), mouse (Q9Z2W0). Other species not tested yet.
Specificity	Shows band of expected size in westernblots. Specifically detects GFP-fusion protein.
Matching control protein/peptide	296-0P
Data sheet	296_003.pdf

Cat. No.: 296 003

Amount: 50 µg

Price: $375.00

Background

Aspartyl aminopeptidase (ASPEP), also referred to as DAP and DNPEP, is an ubiquitously expressed protein that probably plays an important role in intracellular protein and peptide metabolism.

1 ) Chen Y et al., J. Biol. Chem. (2012)
2 ) Nakamura Y et al., Mol. Cell. Biol. (2011)
3 ) Cai WW et al., Biosci. Biotechnol. Biochem. (2010)
4 ) Wilk S et al., J. Biol. Chem. (1998)

Protocols

OTHER

Preabsorbtion

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