Synaptic Systems

Cat. No.: 155 003

Amount: 50 µg

Price: $455.00

Cat. No. 155 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 1000 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)
Reactivity	Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog. Other species not tested yet.
Specificity	Specific for AP 180. K.O. validated PubMed: 26412491
Matching control protein/peptide	155-0P
Data sheet	155_003.pdf

References for AP180 - 155 003

Physical exercise mediates cortical synaptic protein lactylation to improve stress resilience.
Yan L, Wang Y, Hu H, Yang D, Wang W, Luo Z, Wang Y, Yang F, So KF, Zhang L
Cell metabolism (2024) 369: 2104-2117.e4. 155 003 WB, IP, IHC; tested species: mouse

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 155 003 WB; KO verified; tested species: mouse

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 155 003 WB, IHC; tested species: mouse

Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 155 003 WB; KO verified; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 155 003 WB, ICC, IHC; tested species: mouse,rat

Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 155 003 WB

Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.
Gimber N, Tadeus G, Maritzen T, Schmoranzer J, Haucke V
Nature communications (2015) 6: 8392. 155 003 ICC; tested species: mouse

Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition.
Geis C, Weishaupt A, Hallermann S, Grünewald B, Wessig C, Wultsch T, Reif A, Byts N, Beck M, Jablonka S, Boettger MK, et al.
Brain : a journal of neurology (2010) 13311: 3166-80. 155 003 ICC

Quantitative Fluorescent in situ Hybridization Reveals Differential Transcription Profile Sharpening of Endocytic Proteins in Cochlear Hair Cells Upon Maturation.
Huang G, Eckrich S
Frontiers in cellular neuroscience (2021) 15: 643517. 155 003 IHC; tested species: mouse

AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells.
Kroll J, Özçete ÖD, Jung S, Maritzen T, Milosevic I, Wichmann C, Moser T
Journal of cell science (2020) 1332: . 155 003 IHC; KO verified; tested species: mouse

Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 155 003 IHC; tested species: rat

Cat. No.: 155 003

Amount: 50 µg

Price: $455.00

ICC

IHC

Background

During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of e.g. amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
AP 180 also known as pp155, NP185, F1-20, and SNAP 91 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats.

1 ) Bao H et al., J. Neurophysiol. (2005)
2 ) Yao PJ et al., J. Comp. Neurol. (2005)
3 ) Yao PJ et al., J. Comp. Neurol. (2002)
4 ) Kalthoff C et al., J. Biol. Chem. (2002)
5 ) Yao PJ et al., Neuroscience (1999)
6 ) Hao W et al., J. Biol. Chem. (1999)
7 ) Morris SA et al., EMBO J. (1993)
8 ) Prasad K et al., Biochemistry (1988)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

AP180 antibody - 155 003 K.O.

References for AP180 - 155 003