Synaptic Systems - Ankyrin G

Cat. No.: 386 005

Amount: 50 µg

Price: $465.00

Cat. No. 386 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity	Reacts with: rat (O70511-1), mouse (G5E8K5-1). Other species not tested yet.
Specificity	Specific for Ankyrin G, detects all described splice variants.
Data sheet	386_005.pdf

References for Ankyrin G - 386 005

The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling.
Dorrego-Rivas A, Ezan J, Moreau MM, Poirault-Chassac S, Aubailly N, De Neve J, Blanchard C, Castets F, Fréal A, Battefeld A, Sans N, et al.
Science advances (2022) 836: eabo6333. 386 005 WB, IP

Integrative multi-omic analysis reveals conserved cell-projection deficits in human Down syndrome brains.
Rastogi M, Bartolucci M, Nanni M, Aloisio M, Vozzi D, Petretto A, Contestabile A, Cancedda L
Neuron (2024) 11215: 2503-2523.e10. 386 005 ICC; tested species: mouse

Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 386 005 ICC; tested species: rat

Adaptor protein-3 produces synaptic vesicles that release phasic dopamine.
Jain S, Yee AG, Maas J, Gierok S, Xu H, Stansil J, Eriksen J, Nelson AB, Silm K, Ford CP, Edwards RH, et al.
Proceedings of the National Academy of Sciences of the United States of America (2023) 12042: e2309843120. 386 005 ICC; tested species: mouse

Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.
Vuong JK, Ergin V, Chen L, Zheng S
Nature communications (2022) 131: 2081. 386 005 ICC; tested species: mouse

14-3-3 proteins stabilize LGI1-ADAM22 levels to regulate seizure thresholds in mice.
Yokoi N, Fukata Y, Okatsu K, Yamagata A, Liu Y, Sanbo M, Miyazaki Y, Goto T, Abe M, Kassai H, Sakimura K, et al.
Cell reports (2021) 3711: 110107. 386 005 ICC; tested species: mouse

Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Miyazaki Y, Otsuka T, Yamagata Y, Endo T, Sanbo M, Sano H, Kobayashi K, Inahashi H, Kornau HC, Schmitz D, Prüss H, et al.
Cell reports (2024) 431: 113634. 386 005 IHC; tested species: mouse

The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels.
Ramirez-Franco J, Debreux K, Sangiardi M, Belghazi M, Kim Y, Lee SH, Lévêque C, Seagar M, El Far O
Neurobiology of disease (2024) : 106513. 386 005 IHC; tested species: mouse

Rescue of Normal Excitability in LGI1-Deficient Epileptic Neurons.
Extrémet J, Ramirez-Franco J, Fronzaroli-Molinieres L, Boumedine-Guignon N, Ankri N, El Far O, Garrido JJ, Debanne D, Russier M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4350: 8596-8606. 386 005 IHC; tested species: mouse

Cat. No.: 386 005

Amount: 50 µg

Price: $465.00

ICC

IHC

IHC-P

Background

Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).

1 ) Fujiwara Y et al., Sci Rep (2016)
2 ) Yang Y et al., J. Cell Biol. (2007)
3 ) Zhou D et al., J. Cell Biol. (1998)
4 ) Wood SJ et al., J. Cell Biol. (1998)
5 ) Kordeli E et al., J. Biol. Chem. (1995)

Protocols

Standard protocol

ICC

IHC

IHC-P

Other products for this target

Ankyrin G antibody - 386 005

References for Ankyrin G - 386 005