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Amphiphysin antibody - 120 003 K.D.

Amphiphysin is a nerve-terminal protein that binds clathrin, dynamin, and synaptojanin
Rabbit polyclonal purified antibody
Cat. No.: 120 003
Amount: 50 µg
Price: $455.00
Cat. No. 120 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)
Reactivity Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken.
Other species not tested yet.
Specificity K.D. validated PubMed: 37243578
Matching control protein/peptide 120-0P
Data sheet 120_003.pdf

References for Amphiphysin - 120 003

The phospho-regulated amphiphysin/endophilin interaction is required for synaptic vesicle endocytosis.
Kontaxi C, Kim N, Cousin MA
Journal of neurochemistry (2023) 1662: 248-264. 120 003 ICC; KD verified; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 120 003 DNA-PAINT; tested species: rat
Cat. No.: 120 003
Amount: 50 µg
Price: $455.00
The phospho-regulated amphiphysin/endophilin interaction is required for synaptic vesicle endocytosis.
Kontaxi C, Kim N, Cousin MA
Journal of neurochemistry (2023) 1662: 248-264. 120 003 ICC; KD verified; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 120 003 DNA-PAINT; tested species: rat
Background

Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.