Synaptic Systems - Amphiphysin

Cat. No.: 120 002

Amount: 200 µl

Price: $360.00

Cat. No. 120 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 400 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)
Reactivity	Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken. Other species not tested yet.
Matching control protein/peptide	120-0P
Data sheet	120_002.pdf

References for Amphiphysin - 120 002

A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 120 002 WB; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 120 002 WB; tested species: mouse

Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity.
Basheer R, Brown R, Ramesh V, Begum S, McCarley RW
Journal of neuroscience research (2005) 825: 650-8. 120 002 WB

SCAMP1 function in endocytosis.
Fernández-Chacón R, Achiriloaie M, Janz R, Albanesi JP, Südhof TC
The Journal of biological chemistry (2000) 27517: 12752-6. 120 002 WB

Trafficking proteins show limited differences in mobility across different postsynaptic spines.
Mougios N, Opazo F, Rizzoli SO, Reshetniak S
iScience (2023) 262: 105971. 120 002 ICC; tested species: rat

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 120 002 ICC; tested species: mouse

Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.
von Kleist L, Stahlschmidt W, Bulut H, Gromova K, Puchkov D, Robertson MJ, MacGregor KA, Tomilin N, Tomlin N, Pechstein A, Chau N, et al.
Cell (2011) 1463: 471-84. 120 002 ICC; tested species: mouse

A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration.
Scott DA, Tabarean I, Tang Y, Cartier A, Masliah E, Roy S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3024: 8083-95. 120 002 ICC

Loss of the parkinsonism-associated protein FBXO7 in glutamatergic forebrain neurons in mice leads to abnormal motor behavior and synaptic defects.
Wang J, Joseph S, Vingill S, Dere E, Tatenhorst L, Ronnenberg A, Lingor P, Preisinger C, Ehrenreich H, Schulz JB, Stegmüller J, et al.
Journal of neurochemistry (2023) : . 120 002 IHC; tested species: mouse

Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 120 002 IHC, EM; tested species: rat

Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.
Neef J, Jung S, Wong AB, Reuter K, Pangrsic T, Chakrabarti R, Kügler S, Lenz C, Nouvian R, Boumil RM, Frankel WN, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 343: 705-16. 120 002 IHC; tested species: mouse

Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 120 002 IHC

Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 120 002

Cat. No.: 120 002

Amount: 200 µl

Price: $360.00

SCAMP1 function in endocytosis.
Fernández-Chacón R, Achiriloaie M, Janz R, Albanesi JP, Südhof TC
The Journal of biological chemistry (2000) 27517: 12752-6. 120 002 WB

ICC

IHC

Background

Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.

1 ) Slepnev VI et al., Nat. Rev. Neurosci. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Marsh M et al., Science (1999)
4 ) Wigge P et al., Trends Neurosci. (1998)
5 ) Wigge P et al., Mol. Biol. Cell (1997)
6 ) David C et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
7 ) Südhof TC et al., Nature (1995)
8 ) De Camilli P et al., J. Exp. Med. (1993)
9 ) Lichte B et al., EMBO J. (1992)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Amphiphysin antibody - 120 002

References for Amphiphysin - 120 002