Synaptic Systems

Cat. No.: 182 011

Amount: 100 µg

Price: $435.00

Cat. No. 182 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 200 gallery IHC: 1 : 200 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	160E5
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 895 to 907 from rat GluA1 (UniProt Id: P19490)
Reactivity	Reacts with: human (P42261), rat (P19490), mouse (P23818). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	182-01P
Remarks	WB: This antibody is less sensitive than the rabbit polyclonal antibody (cat. no. 182 003).
Data sheet	182_011.pdf

References for GluA1 - 182 011

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 182 011 WB, IHC; tested species: mouse

CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Zhu ZA, Li YY, Xu J, Xue H, Feng X, Zhu YC, Xiong ZQ
Cell reports (2023) 4210: 113202. 182 011 WB; tested species: mouse

PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus.
Lee DS, Kim TH, Park H, Kim JE
Scientific reports (2023) 131: 13927. 182 011 WB; tested species: mouse

AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats.
Kim JE, Lee DS, Park H, Kim TH, Kang TC
Biomedicines (2021) 98: . 182 011 WB, IP; tested species: rat

ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 182 011 WB; tested species: mouse

Chronic mild corticosterone exposure during adolescence enhances behaviors and upregulates neuroplasticity-related proteins in rat hippocampus.
Li J, Li Y, Sun Y, Wang H, Liu X, Zhao Y, Wang H, Su Y, Si T
Progress in neuro-psychopharmacology & biological psychiatry (2019) 89: 400-411. 182 011 WB; tested species: rat

Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 182 011 WB; tested species: mouse

Hippocampal Memory Recovery After Acute Stress: A Behavioral, Morphological and Molecular Study.
Aguayo FI, Tejos-Bravo M, Díaz-Véliz G, Pacheco A, García-Rojo G, Corrales W, Olave FA, Aliaga E, Ulloa JL, Avalos AM, Román-Albasini L, et al.
Frontiers in molecular neuroscience (2018) 11: 283. 182 011 WB; tested species: rat

Heterogeneity of Cell Surface Glutamate and GABA Receptor Expression in Shank and CNTN4 Autism Mouse Models.
Heise C, Preuss JM, Schroeder JC, Battaglia CR, Kolibius J, Schmid R, Kreutz MR, Kas MJH, Burbach JPH, Boeckers TM
Frontiers in molecular neuroscience (2018) 11: 212. 182 011 WB; tested species: mouse

STIM2 regulates AMPA receptor trafficking and plasticity at hippocampal synapses.
Yap KA, Shetty MS, Garcia-Alvarez G, Lu B, Alagappan D, Oh-Hora M, Sajikumar S, Fivaz M
Neurobiology of learning and memory (2017) 138: 54-61. 182 011 WB

Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats.
Pacheco A, Aguayo FI, Aliaga E, Muñoz M, García-Rojo G, Olave FA, Parra-Fiedler NA, García-Pérez A, Tejos-Bravo M, Rojas PS, Parra CS, et al.
Frontiers in molecular neuroscience (2017) 10: 244. 182 011 WB; tested species: rat

Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 182 011 WB; tested species: rat

Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 182 011 WB, ICC

Synaptic function of nicastrin in hippocampal neurons.
Lee SH, Sharma M, Südhof TC, Shen J
Proceedings of the National Academy of Sciences of the United States of America (2014) 11124: 8973-8. 182 011 WB; tested species: mouse

Molecular Characterization of AMPA-Receptor-Containing Vesicles.
Peters JJ, Leitz J, Oses-Prieto JA, Burlingame AL, Brunger AT
Frontiers in molecular neuroscience (2021) 14: 754631. 182 011 IP; tested species: mouse

Abl2 Kinase Differentially Regulates iGluRs Current Activity and Synaptic Localization.
Kabirova M, Reichenstein M, Borovok N, Sheinin A, Gorobets D, Michaelevski I
Cellular and molecular neurobiology (2023) 436: 2785-2799. 182 011 ICC; tested species: rat

Copine-6 is a Ca2+ sensor for activity-induced AMPA receptor exocytosis.
Tan JZA, Jang SE, Batallas-Borja A, Bhembre N, Chandra M, Zhang L, Guo H, Ringuet MT, Widagdo J, Collins BM, Anggono V, et al.
Cell reports (2023) 4212: 113460. 182 011 ICC; tested species: rat

PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 182 011 ICC; tested species: mouse

Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 182 011 ICC; tested species: rat

Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation.
Deane EC, Ilie AE, Sizdahkhani S, Das Gupta M, Orlowski J, McKinney RA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 332: 595-610. 182 011 ICC

Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 182 011 ICC

SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration.
Thumu SCR, Jain M, Soman S, Das S, Verma V, Nandi A, Gutmann DH, Jayaprakash B, Nair D, Clement JP, Marathe S, et al.
eLife (2024) 13: . 182 011 IHC; tested species: mouse

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 182 011 WB, IHC; tested species: mouse

Loss of miR-183/96 alters synaptic strength via pre- and postsynaptic mechanisms at a central synapse.
Krohs C, Körber C, Ebbers L, Altaf F, Hollje G, Hoppe S, Dörflinger Y, Prosser HM, Nothwang HG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 182 011 IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 182 011 DNA-PAINT; tested species: rat

Cat. No.: 182 011

Amount: 100 µg

Price: $435.00

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 182 011 WB, IHC; tested species: mouse

ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.
Nyitrai H, Wang SSH, Kaeser PS
Cell reports (2020) 3110: 107712. 182 011 WB; tested species: mouse

Kibra Modulates Learning and Memory via Binding to Dendrin.
Ji Z, Li H, Yang Z, Huang X, Ke X, Ma S, Lin Z, Lu Y, Zhang M
Cell reports (2019) 268: 2064-2077.e7. 182 011 WB; tested species: mouse

Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 182 011 WB, IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN and kainate/GluK receptors (KARs). mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins.
Glutamate receptors of the AMPA subtype are monovalent cation channels and are composed of the four AMPA subunits GluA 1, GluA 2, GluA 3, and GluA 4.

1 ) Collingridge GL et al., Neuropharmacology (2009)
2 ) Prithviraj R et al., Dev Neurobiol (2008)
3 ) Andin J et al., Brain Res. (2007)
4 ) Lee HK et al., Mol. Cell. Neurosci. (2007)
5 ) Pei W et al., J. Biol. Chem. (2007)
6 ) Deng YP et al., J. Chem. Neuroanat. (2007)
7 ) Nagy GG et al., J. Neurosci. (2004)
8 ) Lee HK et al., Cell (2003)
9 ) Lee HK et al., Neuron (1998)
10 ) Hughes TE et al., Vis. Neurosci. (1992)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

GluA1 antibody - 182 011 K.O.

References for GluA1 - 182 011