Aldh1L1 antibody - 278 003

Aldh1L1 is a folate metabolic enzyme

Rabbit polyclonal purified antibody

Cat. No.: 278 003

Amount: 50 µg

Price: $375.00

Cat. No. 278 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 250 to 269 from rat Aldh1L1 (UniProt Id: P28037)
Reactivity	Reacts with: rat (P28037). Other species not tested yet.
Specificity	Specific for Aldh1L1.
Matching control protein/peptide	278-0P
Data sheet	278_003.pdf

References for Aldh1L1 - 278 003

Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 278 003 WB; tested species: rat

Cat. No.: 278 003

Amount: 50 µg

Price: $375.00

Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 278 003 WB; tested species: rat

Background

Aldehyde dehydrogenase family 1 member L1 (Aldh1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH) is a cytosolic enzyme involved in folate metabolism. It has been shown to be involved in the regulation of cell proliferation and is downregulated in malignant human tumors and cancer cell lines.
Aldh1L1 is highly expressed in several cell-types like hepatocytes and astrocytes.

1 ) Oleinik NV et al., Genes Cancer (2011)
2 ) Yang Y et al., Glia (2011)
3 ) Rodriguez FJ et al., J. Neuropathol. Exp. Neurol. (2008)
4 ) Cahoy JD et al., J. Neurosci. (2008)
5 ) Anthony TE et al., J. Comp. Neurol. (2007)
6 ) Krupenko SA et al., Cell Growth Differ. (2002)

Protocols

OTHER

Preabsorbtion

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