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Home > Products > 293 003

ADAR1 p150 antibody - 293 003 K.O.

ADAR1 p150 is an adenosine deaminase acting on RNA
Rabbit polyclonal purified antibody
Cat. No.: 293 003
Amount: 50 µg
Price: $375.00
Cat. No. 293 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not recommended
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 248 from mouse ADAR1p150 (UniProt Id: Q99MU3)
Reactivity Reacts with: rat (P55266), mouse (Q99MU3).
Other species not tested yet.
Specificity Specific for ADAR1 p150. K.O. validated PubMed: 31956894
Data sheet 293_003.pdf

References for ADAR1 p150 - 293 003

Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
ADAR1 prevents autoinflammation by suppressing spontaneous ZBP1 activation.
de Reuver R, Verdonck S, Dierick E, Nemegeer J, Hessmann E, Ahmad S, Jans M, Blancke G, Van Nieuwerburgh F, Botzki A, Vereecke L, et al.
Nature (2022) 6077920: 784-789. 293 003 WB; KO,KD verified; tested species: mouse
ADAR1 interaction with Z-RNA promotes editing of endogenous double-stranded RNA and prevents MDA5-dependent immune activation.
de Reuver R, Dierick E, Wiernicki B, Staes K, Seys L, De Meester E, Muyldermans T, Botzki A, Lambrecht BN, Van Nieuwerburgh F, Vandenabeele P, et al.
Cell reports (2021) 366: 109500. 293 003 WB; tested species: mouse
An internal deletion of ADAR rescued by MAVS deficiency leads to a minute phenotype.
Bajad P, Ebner F, Amman F, Szabó B, Kapoor U, Manjali G, Hildebrandt A, Janisiw MP, Jantsch MF
Nucleic acids research (2020) : . 293 003 WB; KO verified; tested species: mouse
Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
Cat. No.: 293 003
Amount: 50 µg
Price: $375.00
Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
ADAR1 prevents autoinflammation by suppressing spontaneous ZBP1 activation.
de Reuver R, Verdonck S, Dierick E, Nemegeer J, Hessmann E, Ahmad S, Jans M, Blancke G, Van Nieuwerburgh F, Botzki A, Vereecke L, et al.
Nature (2022) 6077920: 784-789. 293 003 WB; KO,KD verified; tested species: mouse
ADAR1 interaction with Z-RNA promotes editing of endogenous double-stranded RNA and prevents MDA5-dependent immune activation.
de Reuver R, Dierick E, Wiernicki B, Staes K, Seys L, De Meester E, Muyldermans T, Botzki A, Lambrecht BN, Van Nieuwerburgh F, Vandenabeele P, et al.
Cell reports (2021) 366: 109500. 293 003 WB; tested species: mouse
An internal deletion of ADAR rescued by MAVS deficiency leads to a minute phenotype.
Bajad P, Ebner F, Amman F, Szabó B, Kapoor U, Manjali G, Hildebrandt A, Janisiw MP, Jantsch MF
Nucleic acids research (2020) : . 293 003 WB; KO verified; tested species: mouse
Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
Regulation of the double-stranded RNA response through ADAR1 licenses metaplastic reprogramming in gastric epithelium.
Sáenz JB, Vargas N, Cho CJ, Mills JC
JCI insight (2022) 73: . 293 003 WB, IHC, IHC-P; tested species: mouse
WB
WB
Background

ADARs bind to double stranded RNA regions and deaminate adenosine residues to inosine. An inosine is interpreted as a guanosine by the translation machinery leading to alterations of codons.
In addition microRNAs involved in posttranscriptional regulation are modulated through ADAR mediated RNA editing.
Three members of the ADAR gene family (ADAR 1-3) have been identified in vertebrates. In addition, two isoforms of ADAR1 are synthesized by translation initiation at alternative start codons, an interferon-inducible, cytoplasmic 150-kDa protein (p150) and a constitutive, nuclear 110-kDa protein (p110).