ADAM22 antibody - 317 005

ADAM22 is an ADAM family member involved in synaptic signal transmission

Guinea pig polyclonal purified antibody

Cat. No.: 317 005

Amount: 50 µg

Price: $460.00

Cat. No. 317 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 775 to 905 from mouse ADAM22 (UniProt Id: Q9R1V6)
Reactivity	Reacts with: rat, mouse (Q9R1V6). Other species not tested yet.
Specificity	Specific for ADAM 22.
Data sheet	317_005.pdf

Cat. No.: 317 005

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

ADAMs (a disintegrin and metalloproteinases) are a family of multidomain transmembrane glycoproteins.
With few exceptions most ADAM proteins are active zinc metalloproteinases. Members that lack functional metalloproteinase domains are implicated in protein-protein interactions instead of membrane protein ectodomain shedding. ADAM 22, one of these non-proteinase ADAMs acts as a receptor on the surface of the postsynaptic neuron and is involved in the regulation of synaptic signal transmission.

1 ) Bolger JC et al., Vitam. Horm. (2013)
2 ) Ozkaynak E et al., J. Neurosci. (2010)
3 ) Ogawa Y et al., J. Neurosci. (2010)
4 ) Liu H et al., J. Biol. Chem. (2009)

Protocols

ICC

IHC