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Acetylcholinesterase (AChE) antibody - 425 006

AChE Terminates the signal transmission at the cholinergic synapses
Chicken polyclonal purified antibody
Cat. No.: 425 006
Amount: 200 µl
Price: $380.00
Cat. No. 425 006 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 250 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136)
Reactivity Reacts with: rat (P37136), mouse (P21836).
Other species not tested yet.
Specificity Detects isoform T, may cross react to isoforms R and H.
Remarks

IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.
IHC-P: Antigen retrieval (10mM Tris, 1mM EDTA, pH 9.0) is recommended.

Data sheet 425_006.pdf
Cat. No.: 425 006
Amount: 200 µl
Price: $380.00
Background

Acetylcholinesterase also known as AChE or Acetylhydrolase, hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and cholinergic synapses in the brain and thus terminates signal transmission. The protein is also found on the membranes of erythrocytes.

Acetylcholinesterase is a globular, glycosylated protein that lacks a transmembrane peptide-anchor region and occurs as several isoforms, originating from alternative splicing of the AChE mRNA.