Cat. No.: 425 006
Amount: 200 µl
Price:
$380.00
Cat. No. 425 006 |
200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 100 up to 1 : 250 (see remarks) gallery |
Immunogen | Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136) |
Reactivity |
Reacts with: rat (P37136), mouse (P21836). Other species not tested yet. |
Specificity | Detects isoform T, may cross react to isoforms R and H. |
Remarks |
IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended. |
Data sheet | 425_006.pdf |
Acetylcholinesterase also known as AChE or Acetylhydrolase, hydrolyzes the neurotransmitter acetylcholine at neuromuscular junctions and cholinergic synapses in the brain and thus terminates signal transmission. The protein is also found on the membranes of erythrocytes.
Acetylcholinesterase is a globular, glycosylated protein that lacks a transmembrane peptide-anchor region and occurs as several isoforms, originating from alternative splicing of the AChE mRNA.