Synaptic Systems

Cat. No.: 218 903

Amount: 50 µg

Price: $375.00

Immunohistochemical analysis of spinal cord components in mouse model of experimental autoimmune encephalomyelitis.
Pyka-Fosciak G, Stasiolek M, Litwin JA
Folia histochemica et cytobiologica (2018) 563: 151-158. 218 903 IHC; tested species: mouse

WB

IHC

IHC-P

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.

1 ) Marcello A et al., J Neural Transm (Vienna) (2009)
2 ) Baril L et al., Neurosci. Lett. (2004)
3 ) Bayer TA et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Hsiao K et al., Science (1996)
5 ) Wood SJ et al., J. Mol. Biol. (1996)
6 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

WB

IHC

IHC-P

Cat. No. 218 903	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (ECL detection) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 100 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 38 to 43 from human Abeta43 (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), rat (P08592), mouse (P12023). Other species not tested yet.
Specificity	Specific for Abeta 43
Remarks	WB: Detects purified Abeta 43. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min. IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with formic acid is required.
Data sheet	218_903.pdf

Abeta43 antibody - 218 903

References for Abeta43 - 218 903