Synaptic Systems - Abeta38/40/42/43

Cat. No.: 218 711

Amount: 100 µg

Price: $415.00

Female Mice with Apolipoprotein E4 Domain Interaction Demonstrated Impairments in Spatial Learning and Memory Performance and Disruption of Hippocampal Cyto-Architecture.
Adeosun SO, Hou X, Shi L, Stockmeier CA, Zheng B, Raffai RL, Weisgraber KH, Mosley TH, Ming Wang J
Neurobiology of learning and memory (2019) : . 218 711 Dot blot; tested species: mouse

IHC

IHC-P

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42/43 and additional C- and N-terminally modified fragments of Abeta as for instance Abeta pE3 and Abeta pE11.
An additional Abeta variant, Abeta38, is more soluble compared to other Abeta species and is not found in plaques of sporadic Alzheimer´s cases. However, it is detected in the blood-vessel walls of a subset of patients with severe cerebral amyloid angiopathy. It especially accumulates in brains of patients carrying mutations in the Abeta coding region.
Cleavage of amyloid precursor protein APP by β- and γ- secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents the formation of Abeta from APP.

1 ) Marcello A et al., J Neural Transm (Vienna) (2009)
2 ) Baril L et al., Neurosci. Lett. (2004)
3 ) Bayer TA et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Hsiao K et al., Science (1996)
5 ) Wood SJ et al., J. Mol. Biol. (1996)
6 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

IHC

IHC-P

Other products for this target

Cat. No. 218 711	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	88B12
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 17 to 26 from human Abeta (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), mouse (P12023). Other species not tested yet.
Specificity	Specific for Abeta 38, 40, 42. Due to sequence homology, a cross-reactivity to unprocessed APP cannot be excluded.
Remarks	IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with formic acid is required.
Data sheet	218_711.pdf

Abeta38/40/42/43 antibody - 218 711

References for Abeta38/40/42/43 - 218 711