gamma SNAP antibody - 112 303

gamma SNAP is a peripheral membrane protein required for membrane traffic

Rabbit polyclonal purified antibody

Cat. No.: 112 303

Amount: 50 µg

Price: $375.00

Cat. No. 112 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 2000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 18 from rat γSNAP (UniProt Id: Q9CWZ7)
Reactivity	Reacts with: human (Q99747), rat (Q9CWZ7), mouse (Q9CWZ7), cow. No signal: zebrafish. Other species not tested yet.
Specificity	Specific for γ SNAP.
Matching control protein/peptide	112-3P
Data sheet	112_303.pdf

References for gamma SNAP - 112 303

Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 112 303 WB

SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming.
Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS, et al.
Neuron (2010) 683: 473-87. 112 303 WB

Cat. No.: 112 303

Amount: 50 µg

Price: $375.00

Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 112 303 WB

Background

The proteins α/β-SNAP are two related soluble and highly conserved proteins that bind to the fusion complex (SNARE complex), thus allowing the N-ethylmaleimide sensitive fusion protein NSF to bind to the complex. γ-SNAP binds directly to NSF and Gaf-1/Rip11, a protein of the Rab11 interacting family. In contrast to α/β-SNAP it does not interact directly with SNARE proteins and is not required for ER-Golgi transport. SNAP-proteins are abundantly expressed in all tissues. They are partially soluble, partially membrane-bound.

1 ) Tani K et al., J. Biol. Chem. (2003)
2 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Peter F et al., J. Cell. Sci. (1998)
5 ) Südhof TC et al., Nature (1995)
6 ) Rothman JE et al., Nature (1994)

Protocols

Standard protocol

OTHER

Preabsorbtion

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