beta SNAP antibody - 112 203

beta-SNAP is a peripheral membrane protein required for membrane traffic

Rabbit polyclonal purified antibody

Cat. No.: 112 203

Amount: 50 µg

Price: $375.00

Cat. No. 112 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 200 up to 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 283 to 298 from mouse βSNAP (UniProt Id: P28663)
Reactivity	Reacts with: human (P60880), rat (P60881), mouse (P28663), cow. Other species not tested yet.
Specificity	Specific for β SNAP.
Matching control protein/peptide	112-2P
Data sheet	112_203.pdf

Cat. No.: 112 203

Amount: 50 µg

Price: $375.00

Background

The proteins α/β-SNAP are two related soluble and highly conserved proteins that bind to the fusion complex (SNARE complex), thus allowing the N-ethylmaleimide sensitive fusion protein NSF to bind to the complex. γ-SNAP binds directly to NSF and Gaf-1/Rip11, a protein of the Rab11 interacting family. In contrast to α/β-SNAP it does not interact directly with SNARE proteins and is not required for ER-Golgi transport. SNAP-proteins are abundantly expressed in all tissues. They are partially soluble, partially membrane-bound.

1 ) Tani K et al., J. Biol. Chem. (2003)
2 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Peter F et al., J. Cell. Sci. (1998)
5 ) Südhof TC et al., Nature (1995)
6 ) Rothman JE et al., Nature (1994)

Protocols

Standard protocol

OTHER

Preabsorbtion

Other products for this target