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beta-Galactosidase antibody - 435 004

beta-Galactosidase is a frequently used reporter protein
Guinea pig polyclonal antiserum
Cat. No.: 435 004
Amount: 100 µl
Price: $365.00
Cat. No. 435 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 1024 from E.coli β-galactosidase (UniProt Id: P00722)
Specificity Specific for β-Galactosidase.
Data sheet 435_004.pdf
Cat. No.: 435 004
Amount: 100 µl
Price: $365.00
Background

Galactosidases are enzymes that catalyze the hydrolysis of galactosides into monosaccharides. They can be classified as α- or β-Galactosidase. In E.coli the latter one is encoded by the Lac-Z gene and is responsible for breaking down the disaccharide lactose into its monosaccharide components, glucose and galactose.

β-Galactosidase can be used to track the efficiency of bacterial transformation with a recombinant plasmid in a process called Blue/White Color Screening. It is also a frequently used reporter gene to monitor tissue specific gene-expression.