anti-Human IgG sdAb - FluoTag-X2 - N4702-AF568-S

Secondary single domain antibody specific for human IgG

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N4702-AF568-S

Amount: 500 µl

Price: $250.00

Cat. No. N4702-AF568-S	500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with AZdye 568. Fluorescence labeled antibodies conjugated to Alexa^® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye^® 568: λ_ex 568 nm / λ_em 665 nm (identical to Alexa^® 568) Alexa^® 647: λ_ex 650 nm / λ_em 665 nm This product or portions thereof is manufactured under license from Life Technologies Corporation. Alexa Fluor is a registered trademark of Life Technologies Corporation. Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation. Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: yes ICC: 1 : 500 gallery IHC: 1 : 500 FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	AZdye 568, two fluorophores coupled to one FluoTag
Clone	2F3
Subtype	single domain
Immunogen	human IgG fraction
Reactivity	Reacts with: human. No signal: mouse, rabbit, Guinea pig, rat, goat, chicken. Other species not tested yet.
Specificity	Recognizes native human IgG1, 3 and 4. It does not detect human IgG2. No significant cross-reactivity to mouse, rabbit, Guinea pig, rat, goat IgGs and chicken IgY.

Cat. No.: N4702-AF568-S

Amount: 500 µl

Price: $250.00

ICC

Background

FluoTag®-X2 anti-Human is a species-specific FluoTag® directed against the IgG1 subtype from Humans. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Human antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.

Protocols

Fluorescent Detection - FluoTags

ICC

NanoTag Multiplexing

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