Synaptic Systems - PSD95 sdAb

Cat. No.: N3702-At488-L

Amount: 200 µl

Price: $515.00

Cat. No. N3702-At488-L	200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO^® 488. Albumin was added for stabilization. For reconstitution add 200 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage	–80°C for up to 12 month
Applications	WB: N/A IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet
Label	ATTO 488, two fluorophores coupled to one FluoTag
Clone	1B2
Subtype	single domain
Immunogen	Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)
Reactivity	Reacts with: mouse (Q62108), rat (P31016), human (P78352). Other species not tested yet.
Matching control protein/peptide	124-01P

References for PSD95 sdAb - N3702-At488-L

IHC (1)

A cross-species proteomic map reveals neoteny of human synapse development.
Wang L, Pang K, Zhou L, Cebrián-Silla A, González-Granero S, Wang S, Bi Q, White ML, Ho B, Li J, Li T, et al.
Nature (2023) 6227981: 112-119. N3702-At488-L IHC; tested species: human

Cat. No.: N3702-At488-L

Amount: 200 µl

Price: $515.00

ICC

IHC

Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

1 ) Fujita A et al., Biochem. Biophys. Res. Commun. (2000)
2 ) Gundelfinger ED et al., Naturwissenschaften (2000)
3 ) Irie M et al., Science (1997)
4 ) Chow RH et al., Neuron (1996)
5 ) Kornau HC et al., Science (1995)

Protocols

Other products for this target

PSD95 sdAb - FluoTag-X2 - N3702-At488-L

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References for PSD95 sdAb - N3702-At488-L