Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

PSD95 sdAb - FluoTag-X2 - N3702-At488-L

PSD 95 is a component of postsynaptic densities in central synapses

This product was developed by   

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3702-At488-L
Amount: 200 µl
Price: $515.00
Cat. No. N3702-At488-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage –80°C for up to 12 month
Applications
 
WB: N/A
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1B2
Subtype single domain
Immunogen Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)
Reactivity Reacts with: mouse (Q62108), rat (P31016), human (P78352).
Other species not tested yet.
Matching control protein/peptide 124-01P

References for PSD95 sdAb - N3702-At488-L

A cross-species proteomic map reveals neoteny of human synapse development.
Wang L, Pang K, Zhou L, Cebrián-Silla A, González-Granero S, Wang S, Bi Q, White ML, Ho B, Li J, Li T, et al.
Nature (2023) 6227981: 112-119. N3702-At488-L IHC; tested species: human

Cat. No.: N3702-At488-L
Amount: 200 µl
Price: $515.00
A cross-species proteomic map reveals neoteny of human synapse development.
Wang L, Pang K, Zhou L, Cebrián-Silla A, González-Granero S, Wang S, Bi Q, White ML, Ho B, Li J, Li T, et al.
Nature (2023) 6227981: 112-119. N3702-At488-L IHC; tested species: human
Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.