Cat. No.: N3501-AF647-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N3501-AF647-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with
Alexa 647.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage | –80°C for up to 12 month |
| Applications |
ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet |
| Label | Alexa 647, one fluorophore coupled to one FluoTag |
| Clone | W25 |
| Subtype | single domain |
| Immunogen | recombinant S1-Spike protein from SARS-CoV-2 (UniProt Id: P0DTC2) |
| Specificity | RBD of S1 protein from SARS-CoV-2 |
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This anti-S1 SARS-CoV-2 nanobody was developed by the team of Alejandro Rojas-Fernandez at the Institute of Medicine from the Universidad Austral de Chile. NanoTag acquired the exclusive license to produce this nanobody and make it available or research and development purposes. The FluoTag-Q anti-S1 SARS-CoV-2 is based on the W25 clone, which has a sub-nanomolar affinity for the SARS-CoV-2 S1 protein receptor binding domain (RBD) and can neutralize infections as it efficiently competes with ACE-2 receptor binding.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.