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mRuby sdAb - N3305-Biotin

Single domain antibody recognizing a pH resistant fluorescent protein

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody
Cat. No.: N3305-Biotin
Amount: 250 µg
Price: $770.00
Cat. No. N3305-Biotin 250 µg purified antibody, lyophilized from PBS, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Albumin was added for stabilization. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
ICC: yes
Label biotin
Clone 1F8
Immunogen Recombinant protein corresponding to AA 1 to 227 from Entacmaea quadricolor mRuby
Specificity Recognizes most common mRuby derivatives
Cat. No.: N3305-Biotin
Amount: 250 µg
Price: $770.00
Background

 mRuby and mRuby derivatives are bright monomeric red fluorescent proteins derived from Entacmaea quadricolar. They show exceptional resistance to denaturation at pH extremes.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.