Cat. No.: N2402-Li680-S
Amount: 500 µl
Price:
$250.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N2402-Li680-S |
500 µl purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to 3 months: –20 °C Up to 12 months: –80 °C or below Protect from light! |
| Applications |
ICC: 1 : 500 IHC: 1 : 500 FACS: yes |
| Label | Licor 680RD, two fluorophores coupled to one FluoTag |
| Clone | 10E10 |
| Subtype | single domain |
| Reactivity |
Reacts with: rabbit. No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Specific for Rabbit IgG, does not cross-react with mouse Guinea pig or chicken immunoglobulins |
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Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.