Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

anti-Rabbit IgG sdAb - FluoTag-X2 - N2402-Li680-S

Secondary single domain antibody specific for rabbit IgG

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N2402-Li680-S
Amount: 500 µl
Price: $250.00
Cat. No. N2402-Li680-S 500 µl purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Applications
 
WB: yes
ICC: 1 : 500
IHC: 1 : 500
FACS: yes
Label Licor 680RD, two fluorophores coupled to one FluoTag
Clone 10E10
Subtype single domain
Reactivity Reacts with: rabbit.
No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Specific for Rabbit IgG, does not cross-react with mouse Guinea pig or chicken immunoglobulins

Cat. No.: N2402-Li680-S
Amount: 500 µl
Price: $250.00
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.