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Home > Products > N2402-At488-S

anti-Rabbit IgG sdAb - FluoTag-X2 - N2402-At488-S

Secondary single domain antibody specific for rabbit IgG

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N2402-At488-S
Amount: 500 µl
Price: $250.00
Cat. No. N2402-At488-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

 Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Applications
 
ICC: 1 : 500
IHC: 1 : 500
FACS: yes
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 10E10
Subtype single domain
Reactivity Reacts with: rabbit.
No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Specific for Rabbit IgG, does not cross-react with mouse Guinea pig or chicken immunoglobulins

References for anti-Rabbit IgG sdAb - N2402-At488-S

The absence of functional bassoon at cone photoreceptor ribbon synapses affects signal transmission at Off cone bipolar cell contacts in mouse retina.
Babai N, von Wittgenstein J, Gierke K, Brandstätter JH, Feigenspan A
Acta physiologica (Oxford, England) (2021) 2313: e13584. N2402-At488-S IHC; tested species: mouse

Cat. No.: N2402-At488-S
Amount: 500 µl
Price: $250.00
The absence of functional bassoon at cone photoreceptor ribbon synapses affects signal transmission at Off cone bipolar cell contacts in mouse retina.
Babai N, von Wittgenstein J, Gierke K, Brandstätter JH, Feigenspan A
Acta physiologica (Oxford, England) (2021) 2313: e13584. N2402-At488-S IHC; tested species: mouse
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.
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