Cat. No.: N2002-AF647-S
Amount: 500 µl
Price:
$250.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N2002-AF647-S |
500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with
Alexa 647.
Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to 3 months: –20 °C Up to 12 months: –80 °C or below Protect from light! |
| Applications |
IHC: 1 : 500 FACS: yes |
| Label | Alexa 647, two fluorophores coupled to one FluoTag |
| Clone | 10A4 |
| Subtype | single domain |
| Reactivity |
Reacts with: mouse. No signal: rabbit, chicken, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Recognizes Mouse IgG1 isotype |
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Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the IgG1 subtype from Mouse. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.