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Home > Products > N2002-AF647-S

anti-Mouse IgG1 sdAb - FluoTag-X2 - N2002-AF647-S

Secondary single domain antibody specific for mouse IgG1

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N2002-AF647-S
Amount: 500 µl
Price: $250.00
Cat. No. N2002-AF647-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.

Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568)
Alexa® 647: λex 650 nm / λem 665 nm

This product or portions thereof is manufactured under license from Life Technologies Corporation. 
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation.

 Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Applications
 
ICC: 1 : 500
IHC: 1 : 500
FACS: yes
Label Alexa 647, two fluorophores coupled to one FluoTag
Clone 10A4
Subtype single domain
Reactivity Reacts with: mouse.
No signal: rabbit, chicken, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Recognizes Mouse IgG1 isotype

Cat. No.: N2002-AF647-S
Amount: 500 µl
Price: $250.00
Background

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the IgG1 subtype from Mouse. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.
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