Synaptic Systems - VGluT1 sdAb

Cat. No.: N1602-AF568-L

Amount: 200 µl

Price: $515.00

Cat. No. N1602-AF568-L	200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with AZdye 568. Albumin was added for stabilization. For reconstitution add 200 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: 1 : 500
Label	AZdye 568, two fluorophores coupled to one FluoTag
Clone	Nb9
Immunogen	Recombinant protein corresponding to AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity	Reacts with: mouse (Q3TXX4), rat (Q62634). Other species not tested yet.
Specificity	Specific for VGluT1. No significant cross-reaction with VGluT2 or VGluT3

References for VGluT1 sdAb - N1602-AF568-L

ICC (1)

A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.
Falkovich R, Danielson EW, Perez de Arce K, Wamhoff EC, Strother J, Lapteva AP, Sheng M, Cottrell JR, Bathe M
Cell reports (2023) 425: 112430. N1602-AF568-L ICC; tested species: rat

Cat. No.: N1602-AF568-L

Amount: 200 µl

Price: $515.00

Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

1 ) Takamori S et al., Nature (2000)
2 ) Bellocchio EE et al., Science (2000)
3 ) Bellocchio EE et al., J. Neurosci. (1998)
4 ) Ni B et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

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VGluT1 sdAb - FluoTag-X2 - N1602-AF568-L

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References for VGluT1 sdAb - N1602-AF568-L