Tailor-made Antibodies
and Tools for Life Science

ALFA sdAb - FluoTag-X2 - N1502-Li800-L

The ALFA tag is a novel peptide tag

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies consist only of one antigen binding site of a Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules. FluoTags carry fluorophore molecules coupled in a site specific manner.

 

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.

Camelid single domain antibody

Cat. No.: N1502-Li800-L
Amount: 200 µl
Price: $515.00
Cat. No. N1502-Li800-L 200 µl purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications
 
WB: yes
ICC: 1 : 500
FACS: yes
Label Licor 800CW, two fluorophores coupled to one FluoTag
Clone 1G5
Subtype single domain
Immunogen Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)
Specificity Recognizes the ALFA tag: SRLEEELRRRLTE

References for ALFA sdAb - N1502-Li800-L

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. N1502-Li800-L WB, Dot blot

Cat. No.: N1502-Li800-L
Amount: 200 µl
Price: $515.00