Tailor-made Antibodies
and Tools for Life Science

ALFA sdAb - FluoTag-X2 - N1502-At488-L

The ALFA tag is a novel peptide tag

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies consist only of one antigen binding site of a Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules. FluoTags carry fluorophore molecules coupled in a site specific manner.

 

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.

Camelid single domain antibody

Cat. No.: N1502-At488-L
Amount: 200 µl
Price: $515.00
Cat. No. N1502-At488-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications
 
ICC: 1 : 1000
IHC: 1 : 1000
IHC-P: 1 : 1000
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1G5
Subtype single domain
Immunogen Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)
Specificity Recognizes the ALFA tag: SRLEEELRRRLTE

References for ALFA sdAb - N1502-At488-L

Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
bioRxiv : the preprint server for biology (2023) : . N1502-At488-L IHC; tested species: drosophila

Cat. No.: N1502-At488-L
Amount: 200 µl
Price: $515.00