Cat. No.: N1302-AF568-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N1302-AF568-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with
AZdye 568.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
FACS: yes |
| Label | AZdye 568, two fluorophores coupled to one FluoTag |
| Clone | 2B12 |
| Immunogen | Recombinant protein corresponding to AA 1 to 232 from sea anemone mScarlet-i |
| Specificity | mScarlet-i and most common RFP derivatives |
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mScarlet-i is a basic, very bright red fluorescent protein, derived from a synthetic construct (1). It is reported to be a rapidly-maturing, constitutively fluorescent monomer with moderate acid sensitivity. It is a useful acceptor in Foerster resonance energy transfer (FRET) based imaging techniques (2).
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.