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Home > Products > N1302-AF568-L

anti-mScarlet-i sdAb - FluoTag-X2 - N1302-AF568-L

A bright monomeric red fluorescent protein tag

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1302-AF568-L
Amount: 200 µl
Price: $515.00
Cat. No. N1302-AF568-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with AZdye 568.

Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568)
Alexa® 647: λex 650 nm / λem 665 nm

This product or portions thereof is manufactured under license from Life Technologies Corporation. 
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation.

 Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
ICC: yes
FACS: yes
Label AZdye 568, two fluorophores coupled to one FluoTag
Clone 2B12
Immunogen Recombinant protein corresponding to AA 1 to 232 from sea anemone mScarlet-i
Specificity mScarlet-i and most common RFP derivatives

Cat. No.: N1302-AF568-L
Amount: 200 µl
Price: $515.00
Background

mScarlet-i is a basic, very bright red fluorescent protein, derived from a synthetic construct (1). It is reported to be a rapidly-maturing, constitutively fluorescent monomer with moderate acid sensitivity. It is a useful acceptor in Foerster resonance energy transfer (FRET) based imaging techniques (2).

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.
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