Cat. No.: N1202-Li680-S
Amount: 500 µl
Price:
$250.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N1202-Li680-S |
500 µl purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to three months: -20°C Up to 12 months: -80°C or below Protect from light! |
| Applications |
ICC: 1 : 500 IHC: 1 : 500 FACS: yes |
| Label | Licor 680RD, two fluorophores coupled to one FluoTag |
| Clone | 1A23 |
| Subtype | single domain |
| Reactivity |
Reacts with: mouse. No signal: chicken, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Recognizes Mouse IgG (Kappa light chain), does not cross-react with rabbit, Guinea pig or chicken immunoglobulins |
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Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the κ light chain of mouse.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.