Tailor-made Antibodies
and Tools for Life Science

anti-Mouse IgG kappa light chain sdAb - FluoTag-X2 - N1202-At488-S

Secondary single domain antibody specific for mouse IgG with a kappa light chain

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1202-At488-S
Amount: 500 µl
Price: $250.00
Cat. No. N1202-At488-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.
Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect form light!
Applications
 
ICC: 1 : 500 gallery  
IHC: 1 : 500
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1A23
Subtype single domain
Reactivity Reacts with: mouse.
No signal: rat, rabbit, goat, Guinea pig, donkey, human, cow, horse, pig.
Other species not tested yet.
Specificity Recognizes all mouse IgG subtypes that contain a κ light chain (most do).
It does not cross-react to rat, rabbit, chicken, Guinea pig, or goat IgGs.

References for anti-Mouse IgG kappa light chain sdAb - N1202-At488-S

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. N1202-At488-S IP

Cat. No.: N1202-At488-S
Amount: 500 µl
Price: $250.00