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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N0702-AF647-S||
500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.
Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy.
AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568)
This product or portions thereof is manufactured under license from Life Technologies Corporation.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500
Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.', $event)" style="cursor: help;">FACS: yes
|Label||Alexa 647, two fluorophores coupled to one FluoTag|
Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
|Specificity||Chicken IgY, does not cross-react with mouse, rabbit, Guinea pig or rat immunglobulins|
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.