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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N0304-AF647-L||
200µl 2.5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
WB: not recommended
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Label||Alexa 647, two fluorophores coupled to two FluoTags each|
|Immunogen||Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)|
|Specificity||Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine|
Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric and fast maturating protein with high photostability. Due to its sensitivity to pH changes it can be used as a biological pH indicator.
In FluoTag-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (less than 1.5 nm), which is ideal for all microscopy techniques.
In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.