Synaptic Systems

Cat. No.: HS-516 003

Amount: 50 µg

Price: $370.00

WB

ICC

IHC

IHC-P

Background

CX3CR1 is a member of the seven-transmembrane G-protein coupled receptor (GPCR) family. Its ligand CX3CL1, also known as fractalkine or neurotactin, is expressed by neurons, dendritic cells and inflamed endothelial cells, among others.
CX3CR1 is expressed by several cell types, including monocytes, macrophages and microglia in the brain, dendritic cells, natural killer (NK) cells and some T lymphocytes. This receptor is crucial for regulating cell adhesion and migration, and plays an important role in immune response and inflammation. Recent literature highlights the importance of the receptor in several diseases, including cardiovascular disorders (1), neurodegenerative diseases e.g. influencing the progression of Alzheimer's disease (2), liver fibrosis (3) and cancer (4). It is a promising therapeutic target not only in cancer but also in chronic kidney disease (5).

1 ) Landsman L et al., Blood (2009)
2 ) Pawelec P et al., Cells (2020)
3 ) Karlmark KR et al., Hepatology (2010)
4 ) Rivas-Fuentes S et al., J Cancer (2021)
5 ) Cormican S et al., Front Immunol (2021)

Cat. No. HS-516 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP-staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 750 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse CX3CR1 (UniProt Id: Q9Z0D9)
Reactivity	Reacts with: mouse (Q9Z0D9). No signal: human (P49238). Other species not tested yet.
Remarks	WB: Non-boiled samples are recommended when running SDS-PAGE. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is recommended for immunohistochemical staining.
Data sheet	hs-516_003.pdf

CX3CR1 antibody - HS-516 003