Synaptic Systems

Cat. No.: HS-515 003

Amount: 50 µg

Price: $370.00

WB

IHC

IHC-P

Background

CD55, also known as Complement Decay-Accelerating Factor or DAF, is a glycosylphosphatidylinositol (GPI)-linked membrane inhibitor of the complement system (1). While humans and other mammalian species have only one DAF gene, the mouse has two separate, highly homologous DAF genes in its genome. One gene encodes a GPI-anchored DAF protein that is similar to human DAF (GPI-DAF), and the other gene encodes a molecule that is incorporated into the membrane via a transmembrane domain (TM-DAF) (2). TM-DAF gene expression is restricted to the testis (2,3) whereas the GPI-DAF molecule is found on leukocytes, erythrocytes and serum-exposed stromal cells. CD55 acts as a complement inhibitor via inactivation of C3 convertases, splitting them into their individual proteins and preventing their assembly (4). In addition, CD55 is a binding partner for CD97 (also known as ADGRE5), which is widely expressed in monocytes, granulocytes, lymphocytes, macrophages, dendritic cells and smooth muscle cells and also in a variety of tumors (4). CD55 is particularly upregulated during inflammatory conditions to prevent tissue damage. Its expression is associated with several diseases, including cancer, malaria, protein-losing enteropathy, paroxysmal nocturnal hemoglobinuria, multiple sclerosis and rheumatoid arthritis (4).

1 ) Dho SH et al., Immune Netw (2018)
2 ) Miwa T et al., Immunology (2001)
3 ) Lin F et al., Immunology (2001)
4 ) Dho SH et al., Immune Netw (2018)

Cat. No. HS-515 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 1250 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues surrounding AA 315 of mouse CD55 (UniProt Id: Q61475)
Reactivity	Reacts with: mouse (Q61475). No signal: rat, human. Other species not tested yet.
Data sheet	hs-515_003.pdf

CD55 antibody mouse specific - HS-515 003