MARCO antibody mouse-specific - HS-499 003

MARCO is a scavenger receptor found on particular macrophage subsets

Rabbit polyclonal purified antibody

Cat. No.: HS-499 003

Amount: 50 µg

Price: $370.00

Cat. No. HS-499 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 250 up to 1 : 500 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 400 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse MARCO (UniProt Id: Q60754)
Reactivity	Reacts with: mouse (Q60754). No signal: rat (M0R9F7), human (Q9UEW3). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining
Data sheet	hs-499_003.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-499 003

Amount: 50 µg

Price: $370.00

IHC

IHC-P

Background

The macrophage receptor with collagenous structure (MARCO) belongs to the class A scavenger receptor (SR-A) family, all of which are transmembrane proteins with a homotrimeric structure (1). MARCO is constitutively expressed by peritoneal macrophages and splenic marginal zone macrophages. Its expression can be induced in other macrophages, such as Kupffer cells or alveolar macrophages, after bacterial infection or intravenous injection of LPS (2). MARCO mediates the binding of pathogens and unopsonized environmental particles, suggesting an important role in host defense (3). In tumor-associated macrophages, MARCO defines a subtype of suppressive TAMs (4) and is considered a potential prognostic and immunotherapeutic biomarker (5).

1 ) Kelley JL et al., Crit Rev Immunol (2014)
2 ) Ito S et al., Arch Histol Cytol (1999)
3 ) Palecanda A et al., J Exp Med (1999)
4 ) Georgoudaki AM et al., Cell Rep (2016)
5 ) Dong Q et al., Int Immunopharmacol (2023)