Synaptic Systems - Galectin-3

Cat. No.: HS-477 017BT

Amount: 100 µg

Price: $465.00

WB

IHC-P

Background

Galectin-3 (Gal-3, Mac-2) is a β-galactoside binding protein that is predominantly located in the cytoplasm and shuttles into the nucleus. In addition, it is secreted to the cell surface and into biological fluids. Depending on its localization, it exerts pleiotropic biological functions like cell growth, cell differentiation, inflammation, fibrogenesis, migration, apoptosis and host defense (1). It is also involved in cancer pathogenesis, proliferation and spreading of metastasis (2). Galectin-3 is highly expressed in the covering epithelia of the digestive tract and the urinary system (3) and plays important roles in the organization of renal and intestinal cells. Furthermore, Galectin-3 has been identified in a variety of other cell types including fibroblasts, keratinocytes, granulocytes, dendritic cells and macrophages (2). Galectin-3 is expressed by certain tissue-resident macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and alveolar macrophages in the lung (3). In the brain, Galectin-3 is abundant in activated microglia but not expressed in resting cells. Following brain injury, Galectin-3 triggers anti-inflammatory properties of microglia (4). In Alzheimer’s disease, Gal-3 expression is strictly associated with microglial cell activation around Aβ plaques. In stroke Gal-3 was found to be expressed primarily in proinflammatory microglial cells (5).

1 ) Dong R et al., Int J Mol Med (2018)
2 ) Dumic J et al., Biochim Biophys Acta (2006)
3 ) Kim H et al., Cell Biol Int (2007)
4 ) Rahimian R et al., Drug Discov Today (2018)
5 ) García-Revilla J et al., Cell Death Dis (2022)

Other products for this target

Cat. No. HS-477 017BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-Staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	SY-17F7
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse Galectin-3 (UniProt Id: P16110)
Reactivity	Reacts with: human ( P05089). Weaker signal: mouse (Q61176). Other species not tested yet.
Data sheet	hs-477_017bt.pdf

Galectin-3 antibody - HS-477 017BT