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VCAM1 antibody - HS-470 004

VCAM1 is expressed by cytokine activated endothelium
Guinea pig polyclonal antiserum
Cat. No.: HS-470 004
Amount: 100 µl
Price: $350.00
Cat. No. HS-470 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 750 gallery  
Immunogen Synthetic peptide corresponding to AA 108 to 128 from mouse Vcam1 (UniProt Id: Q3UPN1)
Reactivity Reacts with: mouse (Q3UPN1), rat.
No signal: human (P19320).
Other species not tested yet.
Specificity Specific for mouse and rat Vcam1 without cross-reactivity to human Vcam1
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet hs-470_004.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-470 004
Amount: 100 µl
Price: $350.00
Background

Vascular cell adhesion molecule 1 (Vcam1 or CD106) is an endothelial adhesion molecule of the Ig gene superfamily, that regulates leukocyte migration from the blood into tissues (1). Expression of Vcam1 is induced on endothelial cells during inflammatory diseases in response to a number of inflammatory stimuli, including TNFα, lipopolysaccharide or high levels of reactive oxygen species (2). Vcam1 expression in hematopoietic cells is restricted to cells confined to tissues e.g., red pulp macrophages and follicular dendritic cells in the spleen, whereas circulating cells are virtually devoid of Vcam1 (3). Vcam1 has been found to be implicated in various immunological disorders, including rheumatoid arthritis, transplant rejection and cancer and is therefore a potential therapeutic target (4).