VCAM1 antibody mouse specific - HS-470 003

VCAM1 is expressed by cytokine activated endothelium

Rabbit polyclonal purified antibody

Cat. No.: HS-470 003

Amount: 50 µg

Price: $370.00

Cat. No. HS-470 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 1000 up to 1 : 2000 gallery ELISA: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 108 to 128 from mouse Vcam1 (UniProt Id: Q3UPN1)
Reactivity	Reacts with: mouse (Q3UPN1). No signal: rat (P29534 ), human (P19320). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining
Data sheet	hs-470_003.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-470 003

Amount: 50 µg

Price: $370.00

IHC

IHC-P

Background

Vascular cell adhesion molecule 1 (VCAM1 or CD106) is an endothelial adhesion molecule of the Ig gene superfamily, that regulates leukocyte migration from the blood into tissues (1). Expression of VCAM1 is induced on endothelial cells during inflammatory diseases in response to a number of inflammatory stimuli, including TNFα, lipopolysaccharide or high levels of reactive oxygen species (2). VCAM1 expression in hematopoietic cells is restricted to cells confined to tissues e.g., red pulp macrophages and follicular dendritic cells in the spleen, whereas circulating cells are virtually devoid of VCAM1 (3). VCAM1 has been found to be implicated in various immunological disorders, including rheumatoid arthritis, transplant rejection and cancer and is therefore a potential therapeutic target (4).

1 ) Cook-Mills JM et al., Mol Immunol (2002)
2 ) Cook-Mills JM et al., Antioxid Redox Signal (2011)
3 ) Ulyanova T et al., Blood (2005)
4 ) Kong DH et al., Int J Mol Sci (2018)

Other products for this target