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Chil3 / YM1 antibody mouse specific - HS-442 117

Chil3/YM1 is a marker protein for murine macrophages
Rat monoclonal purified IgG
Cat. No.: HS-442 117
Amount: 100 µg
Price: $415.00
Cat. No. HS-442 117 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 2500 gallery  
IHC-P: 1 : 1000 up to 1 : 4000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Clone SY-301F10
Subtype IgG2a (λ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 280 of mouse Chil3 (UniProt Id: O35744)
Reactivity Reacts with: mouse (O35744).
No signal: human.
Other species not tested yet.
Specificity Specific for mouse Chil3 / YM-1; no cross-reactivity with Chil4 / YM-2
Data sheet hs-442_117.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-442 117
Amount: 100 µg
Price: $415.00
Background

Chitinase-3-like protein 3, also referred to as  Chil 3, Ym1 and ECF-L, is a secreted protein that is primarily produced by macrophages during inflammation. It is only expressed in rodents, but expression profile and domain organization suggest a functional overlap with human AMCase and SI-CLP. Chil 3 has chemotactic activity for eosinophils, T-cells and polymorphonuclear leukocytes, and plays a role in the inflammatory response elicited by parasitic infections, lung disorders and allergy.

This lectin binds chitin, heparin and GlcN oligomers. Although Chil 3 shares homology with chitinase family proteins, it has no enzymatic activity.