Synaptic Systems

Cat. No.: HS-427 117

Amount: 100 µg

Price: $415.00

IHC-P

Background

CD45, also designated as Leukocyte Common Antigen (LCA) or protein tyrosine phosphatase receptor type C (PTPRC), is a type I transmembrane protein expressed on all nucleated cells of the hematopoietic system, except mature erythrocytes and platelets (1). Several isoforms of CD45 have been identified which are generated by differential splicing of exons 4, 5, and 6, thereby generating the CD45RA, RB and RC isoforms, respectively (2). Expression of the isoforms differs according to cell type and functional status. The CD45RABC (B220) long isoform is almost exclusively expressed on B cells (3). Naïve human T cells express the high molecular weight isoform CD45RA. Activation of T cells and differentiation to memory T cells is accompanied by exon exclusion for production of the short isoform CD45RO (3). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. Thus, CD45-deficient humans and mice develop a severe-combined immunodeficiency (SCID) phenotype (4). In macrophages, CD45 plays a central role in their adhesion, morphology and motility (5).

1 ) Trowbridge IS et al., Annu. Rev. Immunol. (1994)
2 ) Birkeland ML et al., J. Mol. Cell. Immunol. (1988)
3 ) Ledbetter JA et al., Proc. Natl. Acad. Sci. U.S.A. (1988)
4 ) Hermiston ML et al., Annu Rev Immunol (2003)
5 ) McNeill L et al., Immunol. Lett. (2004)

Other products for this target

Cat. No. HS-427 117	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-65C7C1
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of human CD45 (UniProt Id: P08575)
Reactivity	Reacts with: human (P08575). No signal: mouse. Other species not tested yet.
Data sheet	hs-427_117.pdf

CD45 antibody human specific - HS-427 117