Synaptic Systems

Cat. No.: 398 117

Amount: 200 µl

Price: $420.00

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Cat. No. 398 117	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 (see remarks) gallery IHC-P (FFPE): 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	311H2
Subtype	IgG2b (λ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 1240 of mouse Ki67 (UniProt Id: E9PVX6)
Reactivity	Reacts with: mouse (E9PVX6). No signal: human (P46013), rat (D4A0Y6), chicken, zebrafish. Other species not tested yet.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is required.
Data sheet	Data Sheet 398_117 Safety Data Sheet (SDS)

References for Ki67 - 398 117

Neuronal MCT2 promotes angiogenesis via lactate in the developing mouse neocortex.
Lee D, Wu A, Yao L, Satish S, Mei L, Xiong WC
Cell death and differentiation (2025) : . 398 117 IHC; tested species: mouse

Uhrf1 in PDGFRα-lineage cells regulates osteophyte formation in osteoarthritis.
Jono A, Yanagihara Y, Sakai H, Saeki N, Kutsuna T, Kinoshita T, Uezumi A, Takao M, Imai Y
iScience (2026) 298: 116927. 398 117 IHC-P (FFPE); tested species: mouse

A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.
Loft A, Schmidt SF, Caratti G, Stifel U, Havelund J, Sekar R, Kwon Y, Sulaj A, Chow KK, Alfaro AJ, Schwarzmayr T, et al.
Cell metabolism (2022) : . 398 117 IHC-P (FFPE); tested species: mouse

Cat. No.: 398 117

Amount: 200 µl

Price: $420.00

Species-specific detection of proliferation antigen-Ki67 in murine but not human FFPE spleen

ICC
(1 img)

IHC
(2 img)

IHC-P (FFPE)
(6 img)

Background

Ki-67 is a nuclear protein which is widely used as a marker of cell proliferation. Its expression is closely associated with the cell cycle: Ki-67 is present in actively cycling cells during the G1, S, G2, and M phases but is generally absent in quiescent cells in the G0 phase. At the cellular level, Ki-67 is predominantly localized in the nucleus during interphase and redistributes to the periphery of condensed chromosomes during mitosis (1,2).
Immunohistochemical staining for Ki-67 is a simple and reproducible method to determine the tumor proliferative index and is a predictive and prognostic biomarker in certain types of human cancer, such as breast cancer, gastric cancer or prostate cancer. In breast cancer higher Ki-67 levels have been associated with an increased risk of disease recurrence (1,3). However, Ki-67 expression is not specific to malignant cells, as it is also present in normally proliferating tissues and activated lymphocytes.

1 ) Scholzen T et al., J. Cell. Physiol. (2000)
2 ) Sun X et al., Chromosoma (2018)
3 ) de Azambuja E et al., Br J Cancer (2007)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Ki67 antibody mouse specific - 398 117 (HS-398 117)

References for Ki67 - 398 117