Synaptic Systems

Cat. No.: HS-397 017BT

Amount: 100 µg

Price: $465.00

WB

IHC-P

Background

The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.

1 ) Gordon S et al., BMC Biol. (2017)
2 ) Gordon S et al., Immunol. Rev. (2014)
3 ) Merad M et al., Annu. Rev. Immunol. (2013)
4 ) Lin HH et al., J. Exp. Med. (2005)
5 ) McKnight AJ et al., J. Leukoc. Biol. (1998)

Other products for this target

Cat. No. HS-397 017BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 (AP-Staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	167B3
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino region of mouse F4/80 unprocessed precursor (UniProt Id: Q61549)
Reactivity	Reacts with: mouse (Q61549). Other species not tested yet.
Data sheet	hs-397_017bt.pdf

F4/80 antibody - HS-397 017BT